Analysis of bacterial growth parttern in bioprocessing of polyhydroxyalkanoates from waste oil by Pseudomonas oleovorans

Pseudomonas oleovorans NCIMB 6576 and Ralstonia eutropha 10442 were used for the production of Polyhydroxyalkanoates (PHA) from industrial waste cooking oils, bacteria cultured on tryptone soya broth (TSB) Tryptone agar (TSA). The growth pattern bacteria, serial dilution viable counting was done using Miles Misra method, 0.5ml (500 µl) sample transferred aseptically into test tubes filled with 4.5ml ringer solution (1/4 strength) resulting in a ten-fold dilution, curve cultures P. NCIMB6576 grown TSB without PS oil shows error bars graph each point depicting standard mean. initial count ranges between 6.37 log10 cfu/ml 5.1 cfu/ml. reached its peak after 30 hours giving approximately 9.7 9.24 as well oil, showing that maximum cell attained at same time. curves TS, where errors depicts means point. start experiments there an 6.1 compared to respectively. It observe time which counts is slightly longer when not carbon source (30 hours) control (27 hours). A decline also noticeable until it reaches minimum value 9.4 log.10 48 experiment involving TS.